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首页> 外文OA文献 >MASSUGU2 Encodes Aux/IAA19, an Auxin-Regulated Protein That Functions Together with the Transcriptional Activator NPH4/ARF7 to Regulate Differential Growth Responses of Hypocotyl and Formation of Lateral Roots in Arabidopsis thaliana
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MASSUGU2 Encodes Aux/IAA19, an Auxin-Regulated Protein That Functions Together with the Transcriptional Activator NPH4/ARF7 to Regulate Differential Growth Responses of Hypocotyl and Formation of Lateral Roots in Arabidopsis thaliana

机译：MASSUGU2编码Aux / IAA19，一种由生长素调节的蛋白，该蛋白与转录激活因子NPH4 / ARF7一起调节拟南芥下胚轴的不同生长反应和侧根的形成。

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摘要

We have isolated a dominant, auxin-insensitive mutant of Arabidopsis thaliana, massugu2 (msg2), that displays neither hypocotyl gravitropism nor phototropism, fails to maintain an apical hook as an etiolated seedling, and is defective in lateral root formation. Yet other aspects of growth and development of msg2 plants are almost normal. These characteristics of msg2 are similar to those of another auxin-insensitive mutant, non-phototropic hypocotyl4 (nph4), which is a loss-of-function mutant of AUXIN RESPONSE FACTOR7 (ARF7) (Harper et al., 2000). Map-based cloning of the MSG2 locus reveals that all four mutant alleles result in amino acid substitutions in the conserved domain II of an Auxin/Indole-3-Acetic Acid protein, IAA19. Interestingly, auxin inducibility of MSG2/IAA19 gene expression is reduced by 65% in nph4/arf7. Moreover, MSG2/IAA19 protein binds to the C-terminal domain of NPH4/ARF7 in a Saccharomyces cerevisiae (yeast) two-hybrid assay and to the whole latter protein in vitro by pull-down assay. These results suggest that MSG2/IAA19 and NPH4/ARF7 may constitute a negative feedback loop to regulate differential growth responses of hypocotyls and lateral root formation.
机译：我们已经分离出一个显性的，对生长素不敏感的拟南芥，massugu2（msg2）突变体，该突变体既不显示下胚轴重力也不能表现出向光性，不能保持根尖钩作为黄化幼苗，并且在侧根形成方面有缺陷。 msg2植物生长和发育的其他方面几乎是正常的。 msg2的这些特征与另一个对生长素不敏感的突变体非光变性下胚轴4（nph4）相似，后者是AUXIN响应因子7（ARF7）的功能丧失突变体（Harper等，2000）。 MSG2基因座的基于图的克隆揭示，所有四个突变体等位基因均导致生长素/吲哚-3-乙酸蛋白IAA19的保守结构域II中的氨基酸取代。有趣的是，在nph4 / arf7中，MSG2 / IAA19基因表达的生长素诱导性降低了65％。此外，MSG2 / IAA19蛋白在酿酒酵母（酵母）双杂交测定中与NPH4 / ARF7的C末端结构域结合，并在体外通过下拉测定法与整个后者蛋白结合。这些结果表明，MSG2 / IAA19和NPH4 / ARF7可能构成负反馈回路，以调节下胚轴和侧根形成的不同生长反应。

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